Introduction

BRAF oncogene is among the most frequently mutated kinases in human cancer. Mutations in codon V600 have been reported in different types of cancers including 40-50% of melanomas, 10-70% of thyroid carcinomas, 10% of colorectal cancers and 3-5% of non- small cell lung cancers (NSCLC).

Most of the BRAF mutations are located in codon 600 and constitute V600E, V600Ec, V600D, V600K, V600R.

BRAF RQ Kit provides ready to use reagents for detection of these five mutations based on Real-Time PCR technology. In this method application of fluorescent probes and analysis of fluorescent kinetics allows detection of amplified product without requiring post-amplification analysis. Therefore, test is accomplished much faster and possibility of contamination with the PCR product is reduced sharply. This kit also includes internal control for preventing false negative results due to failure in DNA extraction or PCR reaction.