Introduction

ETV6-RUNX1 (TEL-AML1) is an abnormality resulted from 21;12 translocation. RUNX1 gene, encodes a protein involved in transcriptional control of hematopoiesis. However, as a result of this transloaction, it is repressed by ETV6-RUNX1 fusion protein. This alteration occurs in approximately 25% of childhood ALL diagnosed between the ages of 2-10 years.

ETV6-RUNX1 (TEL-AML1) RQ provides a ready-to-use system for detection and quantitation of ETV6-RUNX1 transcripts as well as calculation of ETV6-RUNX1 percentage.

This kit is based on Real-Time PCR technology. In this method application of fluorescent probes allows detection of amplified product. Analysis of fluorescent kinetics also leads to detection of the target sequence in the reaction without requiring post- amplification analysis, reducing the possibility of contamination with the PCR product. This kit is designed to be used with RotorGene or StepOne machines.