Introduction

Human Papilloma Virus (HPV) is a Non-enveloped double stranded DNA virus from papillomaviridae family. There are about 150 different types of HPV. HPV infection is considered the main cause of cervical cancer and has been linked to more than 99% of all cases. According to data linking HPV infection to cervical cancer, HPV types have been divided into three groups of High- Risk, Low-Risk and No-Risk. Currently 14 types of 16, 18, 31, 33,

35, 39, 45, 51, 52, 56, 58, 59, 66 and 68 are considered High-

Risk and 6 types considered Low-Risk including 6, 11, 42, 43, 44

and 53.

HPV HR RQ3 kit provides a ready-to-use Real-Time PCR system for detection of HPV DNA including 14 High Risk Types of 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66 and 68 and 2 Low Risk

Types of 6 and 11 with RotorGene or StepOne machines. In this method, application of fluorescent dye labeled probes allows detection of amplified product without requiring post-amplification analysis, reducing the possibility of contamination with the PCR product.

This kit also incorporates an Internal Control (IC) to evaluate DNA extraction quality or PCR inhibition.