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Mouse monoclonal antibody against p63 (Clone ISM013)

Mouse monoclonal antibody against p63 (Clone ISM013)

Product identification

 

ISC107-R3                     3 ml ready-to-use (RTU)

ISC107-R7                     7 ml ready-to-use (RTU)

ISC107-C02                   0.2 ml concentrated

ISC107-C05                   0.5 ml concentrated

Summary and explanation

The p63 gene is located at chromosome 3q27-29 and belongs to the p53 gene family. p63 plays a critical role in the growth and development of many epithelial organs. p63 is confined to basal cells of squamous epithelia (including epidermis and hair follicles) and urothelium, as well as basal cells/myoepithelial cells in breast, sweat glands, salivary glands, and prostate. The expression of p63 has clinical value as a diagnostic marker in various tumour typers. Thus, p63 helps to distinguish squamous cell carcinoma from adenocarcinoma in the lung or urothelial carcinoma from renal cell carcinoma. As a known marker of basal cells in breast and prostate, p63 expression is useful for prostate cancer diagnosis and identifies a basal phenotype in some breast carcinomas

Principle of the procedure

The stated primary antibody is suitable for immunohistochemical staining of FFPE tissue sections based on specific antigen-antibody reaction. Using a detection system linked to HRP or alkaline phosphatase the antigen visualization is performed via specific binding of the primary antibody. Secondary antibody is binding to the primary antibody, and the enzyme complex labels this complex. The enzymatic activation of the chromogen results in a visible reaction product at the antigen site. Each step is incubated for a precise time and temperature and requires interposed washing steps. The specimen may then be counterstained. Results are interpreted using a light microscope.

Staining procedure

  1. Cut 3-4 μm section of formalin-fixed paraffin-embedded tissue and place on positively charged slides.
  2. Dry at 65°C for 2 hours.
  3. Deparaffinize, rehydrate, and epitope retrieve. Upon completion, rinse with 3 changes of distilled or deionized water.
  4. If using HRP detection system, place slides in peroxide block for 10 minutes; rinse. If using AP detection system, omit this step.
  5. Apply the antibody and incubate for 60 minutes; rinse.
  6. Apply the InSituVison™ Polymer Rabbit/Mouse Detection System for 30 minutes; rinse.
  7. Apply ample amount of DAB or AEC chromogen and incubate; rinse.
  8. Dehydrate and coverslip.

Interpretation of results

Warnings and precautions

  1. Application only by qualified and trained personnel.
  2. There are no estimated health risks, if the product is used as directed. MSDS is available on request.
  3. Product contains sodium azide as preservative. Pure sodium azide is toxic. The concentration of sodium azide in this reagent is < 0.1 % and is not classified hazardous.

See MSDS.

  1. As with any product derived from biological sources, proper handling procedures should be used.
  2. Do not use reagents after expiration date.
  3. Take reasonable precautions when handling reagents. Use protective clothing and gloves.
  4. All hazardous materials should be disposed according to guidelines for hazardous waste disposal. Materials of human or animal origin should be handled as biohazardous materials and disposed of with proper precautions.
  5. Avoid microbial contamination of reagents as it may cause incorrect results.

 

Materials provided

Primary antibody p63
Host Mouse
Subclass IgG
Clone ISM013
Species Cross-reactivity Human. Others-not known
Applications Immunohistochemistry
Epitope Retrieval Heat-induced epitope retrieval
Ready-to-use antibody Prediluted antibody in antibody diluent buffer

 

Product label shows the specific lot number.

 

Prediluted antibody is ready-to-use and optimized for staining. No further dilution, reconstitution, mixing, or titration is needed.

Materials required but not provided

The following materials may be required for staining but are not provided with the primary antibody.

– Positive and negative controls

– Microscope slides (positively charged) and cover slips

– Water bath

– Humidified chamber

– Staining jars

– Stopwatch

– Xylene or xylene substitute

– Ethanol

– Deionized or distilled water

– Antigen retrieval reagent, e.g. Antigen Enhancer (HIER buffer), Cat. No. BUAN01-500

– Detection system, e.g. IHC Complete Detection system (Goat anti mouse/rabbit HRP, DAB staining), Cat. No. D001-15

– Wash buffer: e.g. IHC Wash Buffer, Cat. No. BUWA01-1000

– Tap water/bluing reagent (e.g. ammonia water)

– Light microscope

Storage and handling

Store at 2 – 8 °C.

 

When stored correctly antibody is stable to the expiration date indicated on the vial. Do not use after expiration date.

To ensure proper reagent delivery and stability of the antibody, replace the dispenser cap after every use and immediately place the bottle cool in an upright position.

Literature

] Conde E, Angulo B, Redondo P, et al. The use of P63 immunohistochemistry for the identification of squamous cell carcinoma of the lung. PLoS One, 2010, 5(8): e12209.

 

[2] Massion P P, Taflan P M, Rahman S M J, et al. Significance of p63 amplification and overexpression in lung cancer development and prognosis. Cancer research, 2003, 63(21): 7113-7121.

Date of publication or revision

2024-06-10

Change(s) made: –

 

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