Parvovirus B19 RQ kit provides a ready-to-use Real-Time PCR test for the detection and quantitation of Parvovirus B19 DNA using Real-Time PCR (based on polymerase chain reaction) with Rotor- Gene or StepOne machines. All required reagents are included in the PCR Mix provided in the kit. This Mix also contains a different series of primers and probes for the detection of a synthetic DNA sequence. The kit is provided with this synthetic DNA sequence as Internal Control (IC). IC could be either used during the DNA extraction or in the PCR reaction to prevents false negative results due to failure in the above steps.