Introduction

PML-RARA is an abnormality resulted from t(15;17)(q22;q21) translocation. This translocation results in fusion of PML (promyelocytic) gene with RARA (retinoic acid receptor alpha) gene, and production of chimeric PML-RARA protein which is a transcription repressor and impairs the myeloid differentiation. While RARA breakpoints always occure in intron 2, PML breakpoints involves three different regions of intron 6 (55%), exon 6 (5%) and intron 3 (40%). The resulted isoforms of PML- RARA are respectively called bcr1/Long/L, bcr2/Variant/V and bcr3/Short/S.

PML-RARA accounts for more than 90% of APL (acute progranulocytic leukemia) cases, 10-15% of AML (acute myeloid leukemia) cases.

PML-RARA RQ kit provides a ready-to-use system for detection and quantitation of PML-RARA transcripts as well as calculation of PML-RARA percentage.

This kit is based on Real-Time PCR technology. In this method application of fluorescent probes allows detection of amplified product. Analysis of fluorescent kinetics also leads to detection of

the target sequence in the reaction without requiring post- amplification analysis, reducing the possibility of contamination with the PCR product. This kit is designed to be used with RotorGene or StepOne machines.