STI-3 RQ kit provides a ready-to-use Real-Time polymerase chain reaction (PCR) test designed for detecting DNA of the following bacteria: Chlamydia trachomatis, Neisseria gonorrhoeae, and Mycoplasma genitalium. All required reagents are included in the PCR Mix provided in the kit. This Mix also contains a different series of primers and probe for detecting a synthetic DNA sequence. The kit supplies this synthetic DNA sequence as an Internal Control (IC). The IC can be used either during DNA extraction or in the PCR reaction to prevent false negative results due to failure in the above steps.